Brand

MultiSciences

貨號

70-EK106

品名

Human IL-6 ELISA Kit

海關名稱：

Human IL-6 ELISA Kit

適用

ELISA 酶聯免疫吸附測定

反應

Human

測定型

Sandwich ELISA 夾心酶聯免疫分析

Suitable Sample Type

serum, plasma, cell culture supernates

適宜樣本類型

96-well strip plate

貯存

4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)

裝運條件

4℃

取樣容積

50 μl

敏感，感受性

0.37 pg/ml

標準曲線范圍

7.81 - 500 pg/ml

尖峰恢復范圍

99 % - 115 %

平均尖峰恢復

1.06

CV of Intra plate

2.5 % - 5.0 %

CV of Inter plate

1.8 % - 4.4 %

成分

96-well polystyrene microplate coated with a monoclonal antibody against IL-6（96孔聚苯乙烯微板包覆抗IL-6單克隆抗體）

Human IL-6 Standard, lyophilized（人白細胞介素-6標準，凍干）

IL-6 Detect Antibody（IL-6抗體）

Standard Diluent（標準品稀釋液）

Streptavidin-HRP（鏈霉親和素）

Assay Buffer (10×)（分析緩沖液）

Substrate (TMB)（襯底）

Stop Solution（停止液）

washing Buffer (20×)（洗滌緩沖液）

Plate Covers（板蓋）

描述

"This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-6. The Human IL-6 ELISA is for research use only. Not for diagnostic or therapeutic procedures.（該方法采用定量夾心酶免疫分析法對人IL-6進行了定量檢測。人IL-6酶聯免疫吸附試驗僅用于研究.不用于診斷或治療 c程序。）

Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL-6 gene. IL-6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6 also plays a role in fighting infection, as IL-6 has been shown in mice to be required for resistance against bacteriumStreptococcus pneumoniae.

IL-6 stimulates the inflammatory and auto-immune processes in many diseases such as diabetes, atherosclerosis, depression, Alzheimer's Disease, systemic lupus erythematosus, multiple myeloma, prostate cancer, Behçet's disease, and rheumatoid arthritis. Advanced/ metastatic cancer patients have higher levels of IL-6 in their blood."（白細胞介素-6(IL-6)是一種白細胞介素，它既是一種促炎細胞因子，又是一種抗炎性肌因子.在人類中，它是由IL-6基因編碼的。IL-6由T細胞和巨噬細胞分泌。 HAGE刺激免疫應答，例如在感染期間和創傷后，尤其是燒傷或其他組織損傷導致炎癥。IL-6在抵抗感染方面也起作用，因為IL-6具有B 表明小鼠對肺炎鏈球菌的耐藥性是必需的。

IL-6可刺激糖尿病、動脈粥樣硬化、抑郁癥、阿爾茨海默病、系統性紅斑狼瘡、多發性骨髓瘤、前列腺等多種疾病的炎癥和自身免疫過程。 癌癥，貝塞特氏病和類風濕關節炎。晚期/轉移性癌癥患者血液中IL-6水平較高。）