pLVX-IRES-ZsGreen1載體

Description

pLVX-IRES-ZsGreen1 is an HIV-1-based, lentiviral expression vector that allows the simultaneous 

expression of your protein of interest and ZsGreen1 in virtually any mammalian cell type, 

including primary cells. ZsGreen1 is a human codon-optimized variant of the reef coral 

Zoanthus sp. green fluorescent protein, ZsGreen. The vector expresses the two proteins from 

a bicistronic mRNA transcript, allowing ZsGreen1 to be used as an indicator of transduction 

efficiency and a marker for selection by flow cytometry.

Expression of the bicistronic transcript is driven by the constitutively active human 

cytomegalovirus immediate early promoter （P

CMV IE） located just upstream of the MCS. An 

encephalomyocarditis virus （EMCV） internal ribosome entry site （IRES）, positioned between 

the MCS and ZsGreen1, facilitates cap-independent translation of ZsGreen1 from an internal 

start site at the IRES/ZsGreen1 junction （1）

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：楊建輝

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