天天天天天天操 I日韩高清片 I中文字幕首页 I亚洲精品国偷拍自产在线观看蜜桃 I午夜色性片 I婷婷社区五月天 I伊人五月 I五月婷婷在线观看视频 I色偷偷888欧美精品久久久 I色视频在线观看免费 I深夜免费福利在线 I午夜视频导航 I日本婷婷色 I日批视频国产 I天天干.com I亚洲欧洲视频 I亚洲人成人99网站 I天天干 夜夜操 I亚洲热久久 I亚洲综合在线视频 I伊人狠狠色丁香婷婷综合 I中文av一区二区 I夜夜干天天操

您好!歡迎訪問上海起發實驗試劑有限公司網站!
全國服務咨詢熱線:

15921799099

當前位置:首頁 > 資料下載 > pUB6/V5 His B說明書

pUB6/V5 His B說明書

發布時間:2013/8/12      點擊次數:3369

 pUB6/V5 His B

型號 載體名稱 出品公司 載體用途
VPI0023 pUB6/V5 His B Invitrogen 哺乳動物表達載體

 

產品參數: 

Key Function:Constitutive Expression
Mammalian Selection:Blasticidin
Promoter:UbC
Vector Type:pUB6
Cloning Method:Restriction Enzyme/MCS 
Protein Tag or Fusion:V5 Epitope Tag,His Tag (6x His) 
Constitutive or Inducible System:Constitutive

載體抗性: 
氨芐青霉素(Ampicillin)
載體描述: 

pUB6/V5-His A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells.

The vectors contain the following elements:

  1. Human ubiquitin C promoter (hUbC) for high-level expression across a broad range of species and cell types (Schorpp et al., 1996; Wulff et al., 1990).
  2. Three reading frames to facilitate in-frame cloning with a C-terminal peptide encoding the V5 epitope and a polyhistidine (6×His) metal-binding tag.
  3. Blasticidin resistance gene (bsd) for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7).

The control plasmid, pUB6/V5-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

質粒圖譜: 
pUB6/V5-His A&B&C 質粒圖譜
 
上海起福生物科技有限公司
:楊建輝
辦公:
*8006
:
:http://www.bjjmhy.com.cn/
地址:上海市浦東新區繡川路561號1102室

 

 

 


文件下載    
上海起發實驗試劑有限公司
地址:上海浦東川沙鎮川沙路6619號上海起發實驗試劑有限公司
郵箱:xs1@78bio.com
傳真:021-50724961
關注我們
歡迎您關注我們的微信公眾號了解更多信息:
歡迎您關注我們的微信公眾號
了解更多信息